Preanalytical stability of plasma biomarkers for Alzheimer's disease pathology

INTRODUCTION: Plasma tests have demonstrated high diagnostic accuracy for identifying Alzheimer's disease pathology. To facilitate the transition to clinical utility, we assessed whether plasma storage duration and temperature affect the biomarker concentrations. METHODS: Plasma samples from 13 participants were stored at +4°C and +18°C. Concentrations of six biomarkers were measured after 2, 4, 6, 8, 10, and 24 h by single molecule array assays. RESULTS: Phosphorylated tau 181 (p-tau181), phosphorylated tau 231 (p-tau231), neurofilament light (NfL), and glial fibrillary acidic protein (GFAP) concentrations were unchanged both when stored at +4°C and +18°C. Amyloid-β 40 (Aβ40) and amyloid-β 42 (Aβ42) concentrations were stable for 24 h at +4°C but declined when stored at +18°C for longer than 6 h. This decline did not affect the Aβ42/Aβ40 ratio. DISCUSSION: Plasma samples can be stored for 24 h at +4°C or +18°C and result in valid assay results for p-tau181, p-tau231, Aβ42/Aβ40 ratio, GFAP, and NfL. HIGHLIGHTS: Plasma samples were stored for 24 h at +4°C and +18°C, mimicking clinical practice.Concentrations for Alzheimer's disease biomarkers were measured at six time-points.p-tau181, p-tau231, NfL, and GFAP concentrations were unchanged during the experiment.Storage at +18°C affected Aβ40 and Aβ42 concentrations while storage at +4°C did not. The Aβ42/Aβ40 ratio was unaffected.These plasma tests seem suitable for use in general practice

Capillary pressure correlation for mixed-wet reservoirs

Summary For water-wet reservoirs, several expressions may be used to correlate capillary pressure, or height above the free water level, with the water saturation. These correlations all feature a vertical asymptote at the residual water saturation where the capillary pressure goes to plus infinity. We have developed a general capillary pressure correlation that covers primary drainage, imbibition, secondary drainage, and hysteresis scanning loops. The graph exhibits an asymptote at the residual saturation of water and of oil where the capillary pressure goes to plus and minus infinity, respectively. The shape of the correlation is simple yet flexible as a sum of two terms, each with two adjustable parameters and is verified by laboratory experiments and well-log data. An associated hysteresis scheme is also verified by experimental data. The correlation can be used to make representative capillary pressure curves for numerical simulation of reservoirs with varying wettability and to model and interpret flooding processes